Journal: EMBO Molecular Medicine

Article Title: Gut microbiota‐CRAMP axis shapes intestinal barrier function and immune responses in dietary gluten‐induced enteropathy

doi: 10.15252/emmm.202114059

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Article Snippet: In brief, single cell suspensions were stained with biotinylated anti‐CD8a (130‐118‐147), CD11b (130‐113‐233), CD11c (130‐113‐578), CD19 (130‐113‐729), CD45R(B220) (130‐123‐853), CD49b(DX5) (130‐101‐934), CD105 (130‐101‐992), MHC Class II (130‐101‐849), Ter‐119 (130‐120‐828), TCRγ/δ (130‐114‐028) and CD25 (130‐092‐569) followed by streptavidin MACS beads (130‐048‐101) and sorted on an AutoMACS (all Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Isolation, Sequencing, Software, Enzyme-linked Immunosorbent Assay

Journal: Nature Communications

Article Title: Fasting mimicking diet in mice delays cancer growth and reduces immunotherapy-associated cardiovascular and systemic side effects

doi: 10.1038/s41467-023-41066-3

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Article Snippet: Anti-mouse MHC Class II, PE (REA813) , Miltenyi Biotec , 130-112-231.

Techniques: In Vivo

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: PSM Peptides of Staphylococcus aureus Activate the p38-CREB Pathway in Dendritic Cells, Thereby Modulating Cytokine Production and T Cell Priming.

doi: 10.4049/jimmunol.1502232

Figure Lengend Snippet: FIGURE 7. PSMs gain access to the cytosol by transient pore formation. Multispectral imaging flow cytometry analysis of PSMa-FITC+ DCs (for gating, see Supplemental Fig. 4B) 30 min (A) or 10 min (B) after incubation with FITC-labeled PSMa2 (37˚C or ice) in the presence (B) or absence of OVA-Alexa647 (A). (A and B) Representative bright field (BF) and fluorescence images of DCs are shown from two or more independent experiments with similar results performed in triplicate. The graph shows the frequency of PSMa2-FITC+ DCs from WT and FPR22/2 mice. (C) DCs were treated with the indicated reagents for 10 min. The graph shows the frequency of LDH release in relation to DCs treated with 1% Triton-X100 (positive control). The graph shows one of two independent experiments with similar results performed in triplicate (mean 6 SD). ****p , 0.0001, one-way ANOVA with Bonferroni posttest.

Article Snippet: Cells were stained for 20 min at 4 ̊C with extracellular Abs against CD11c-PE (N418; eBioscience) and MHC class II-FITC (M5/114.15.2; Miltenyi).

Techniques: Imaging, Cytometry, Incubation, Labeling, Positive Control

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: PSM Peptides of Staphylococcus aureus Activate the p38-CREB Pathway in Dendritic Cells, Thereby Modulating Cytokine Production and T Cell Priming.

doi: 10.4049/jimmunol.1502232

Figure Lengend Snippet: FIGURE 8. Colocalization of PSMs with p38 and p-p38. Multispectral imaging flow cytometry analysis of DCs (for gating, see Supplemental Fig 4B) 30 min after incubation with FITC-labeled PSMa2 or PSMa3 in the presence or absence of S. aureus cell lysate (sa lysate). Representative bright field (BF) and fluorescence images of PSMa-FITC+ DCs additionally stained with p38 (A) and p-p38 (B). Yellow spots indicate intracellular PSMa2 colocalized with p38 (A) or p-p38 (B). (C) The histogram overlay shows the intensity of p-p38 in DCs treated with PSMa2-FITC (gray) and the combination of S. aureus cell lysate and PSMa2-FITC (red). Numbers in histogram indicate the median intensity of p-p38. Data show representative images from two or more inde- pendent experiments with similar results and performed in triplicate.

Article Snippet: Cells were stained for 20 min at 4 ̊C with extracellular Abs against CD11c-PE (N418; eBioscience) and MHC class II-FITC (M5/114.15.2; Miltenyi).

Techniques: Imaging, Cytometry, Incubation, Labeling, Staining